苯并[a]芘(BaP)对褐菖鲉(Sebasticus marmoratus)肝CYP1A1酶活性、基因表达及蛋白表达的影响     

孙文静  王晓艳  祁鹏志  张建设  吴常文 《海洋与湖沼》2018,49(4):897-903

为了了解苯并[a]芘(BaP)对鱼类细胞色素P4501A1(CYP1A1)表达的影响,以褐菖鲉(Sebasticus marmoratus)为实验材料,采用体内实验,研究其在经过不同浓度(0.1、1、10、20、50mg/kg鱼体重量)的BaP诱导后,鱼体肝脏研究CYP1A1基因表达的情况,筛选出后续时间-效应实验中BaP注射的最佳浓度,研究BaP诱导6h、12h、1d、3d、7d后(质量浓度为20mg/kg鱼体重量)鱼体肝脏CYP1A1酶活性、基因表达和蛋白表达的情况。结果表明:剂量-效应实验中,20mg/kg鱼体重量为最佳浓度,此浓度下,基因表达在各组中变化最显著。时间-效应实验中,较空白对照组而言,染毒6h、12h和1d后,EROD酶活性显著增加。3d后开始下降,与对照组相比变化不大,7d后酶活性又发生上调。半定量RT-PCR结果表明,各染毒组与对照组相比,CYP1A1基因表达量都发生了上调,呈现先上升后下降的趋势。其中,6h和12h组相对表达量极显著增加,1d后开始下降且与3d和7d组相比变化不明显。Western blot结果表明,蛋白表达量在染毒12h后表现出显著的诱导效应,随着时间的延长略有回落,但与对照组相比仍有显著性差异。研究表明:BaP对褐菖鲉CYP1A1具有较强的诱导作用。一定质量浓度的BaP注射于褐菖鲉不同的时间后,能诱导褐菖鲉活体EROD酶活性、CYP1A1基因m RNA表达及蛋白表达,并随着时间的延长呈现先诱导后抑制的趋势。这说明BaP作为诱导剂对CYP1A1酶活性和蛋白表达的作用机制可能与调控CYP1A1的转录水平有关。  相似文献   

Transcriptomic analysis reveals the effect of the exopolysaccharide of Psychrobacter sp. B-3 on gene expression in RAW264.7 macrophage cells     

ZHANG Pingping  LI Jiang  YU Leiye  WEI Jingfang  XU Tong  SUN Guojie 《海洋学报(英文版)》2018,(8)

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印度尼西亚北苏拉威西蓝碧海峡浮游动物种类组成及分布   总被引：1，自引：0，他引：1  

王彦国  陈小银  邢炳鹏  孙柔鑫  FITRIA Nurul  项鹏  王春光  林茂 《海洋学报(英文版)》2018,37(12):35-44

Based on oceanographic survey data in June 2012 in the Lembeh Strait, the zooplankton ecological characteristics such as species composition, individual abundance, dominant species and distribution were analyzed. The results showed that 183 species(including 4 sp.) had been recognized, most of them belonged to copepoda.Cnidaria followed with 43 species(including 1 sp.) were identified. The average abundance of zooplankton was(150.47±58.91) ind./m~3. As to the horizontal distribution, the abundance of the zooplankton was higher in the southern waters than in the northern waters. The dominant species in the study area were Lensia subtiloides,Sagitta enflata, Lucifer intermedius, Oikopleura rufescens, Diphyes chamissoni, Creseis acicula, Subeucalanus subcrassus, Temora discaudata, Aglaura hemistoma, Doliolum denticulatum, Canthocalanus pauper, Oikopleura longicauda and Nanomia bijuga. Zooplankton biodiversity indexes were higher in study area than previous study in the other regions. The findings from this study provide important baseline information for future research and monitoring programs.  相似文献   

Seismogenic Structures of the 2006 ML4.0 Dangan Island Earthquake Offshore Hong Kong     

XIA Shaohong  CAO Jinghe  SUN Jinlong  LV Jinshui  XU Huilong  ZHANG Xiang  WAN Kuiyuan  FAN Chaoyan  ZHOU Pengxiang 《中国海洋大学学报(英文版)》2018,(1)

The northern margin of the South China Sea, as a typical extensional continental margin, has relatively strong intraplate seismicity. Compared with the active zones of Nanao Island, Yangjiang, and Heyuan, seismicity in the Pearl River Estuary is relatively low. However, a ML4.0 earthquake in 2006 occurred near Dangan Island(DI) offshore Hong Kong, and this site was adjacent to the source of the historical M5.8 earthquake in 1874. To reveal the seismogenic mechanism of intraplate earthquakes in DI, we systematically analyzed the structural characteristics in the source area of the 2006 DI earthquake using integrated 24-channel seismic profiles, onshore–offshore wide-angle seismic tomography, and natural earthquake parameters. We ascertained the locations of NW-and NE-trending faults in the DI sea and found that the NE-trending DI fault mainly dipped southeast at a high angle and cut through the crust with an obvious low-velocity anomaly. The NW-trending fault dipped southwest with a similar high angle. The 2006 DI earthquake was adjacent to the intersection of the NE-and NW-trending faults, which suggested that the intersection of the two faults with different strikes could provide a favorable condition for the generation and triggering of intraplate earthquakes. Crustal velocity model showed that the high-velocity anomaly was imaged in the west of DI, but a distinct entity with low-velocity anomaly in the upper crust and high-velocity anomaly in the lower crust was found in the south of DI. Both the 1874 and 2006 DI earthquakes occurred along the edge of the distinct entity. Two vertical cross-sections nearly perpendicular to the strikes of the intersecting faults revealed good spatial correlations between the 2006 DI earthquake and the low to high speed transition in the distinct entity. This result indicated that the transitional zone might be a weakly structural body that can store strain energy and release it as a brittle failure, resulting in an earthquake-prone area.  相似文献   

发现于我国渤海辽东湾的海洋甲藻新种-渤海异冒藻(Heterocapsa bohaiensis sp. nov.)     

肖洁  孙娜  张议文  孙萍  李艳  庞敏  李瑞香 《海洋学报(英文版)》2018,37(10):18-27

A small armed dinoflagellate bloomed in the aquaculture ponds off the coast of Liaodong Bay, Bohai Sea of China,resulting in heavy mortalities of the cultured prawns(Penaeus japonicus) and larvae of Chinese mitten handed crabs(Eriocheir sinensis). The bloom-forming species was successfully isolated, and cellular morphology of the specimen was consequently investigated through light, fluorescent and electron microscopy. The small((14.4±1.6) μm in length) ellipsoid cells show typical Heterocapsa thecal plate arrangement(Po, cp, 5′, 3 a, 7′′, 6 c,5 s, 5′′′, 2′′′′). The episome is evidently bigger than the hyposome. One to three spherical pyrenoids are located above or beside the large elongated nucleus. The body scale is characterized by a triangle basal plate with one central upright and nine peripheral spines. Above all, Heterocapsa bohaiensis could be distinguished from other Heterocapsa species by the combination of the cell size, morphology, cellular structure and body scale. Sequence analyses of both ITS and LSU regions reveal the significant genetic divergence between H. bohaiensis and other established species in this genus, further supporting novelty of this species. Noticeably, different sample treatment methods resulted in morphological variation of the apical pore complex(APC) of H. bohaiensis, which needs to be taken into account in future study.  相似文献   

“蛟龙”号载人潜水器运行与保障信息管理系统设计与应用          下载免费PDF全文

高翔  孙超  丁忠军  宋宏  赵晟娅  祝普强 《海洋开发与管理》2018,35(11):19-26

2017年我国"蛟龙"号载人潜水器完成了为期5年的试验性应用,期间累计下潜100余次,形成和建立了科学完善的应用保障体系和专业的应用人才队伍。以"蛟龙"号试验性应用中运行与保障的实践经验为基础,针对其中设备与备品备件管理、运行作业管理、拆检总装管理、数据资料查询与利用等方面的重要需求,设计开发了一套"蛟龙"号载人潜水器运行与保障信息管理系统,通过信息化平台实现设备资源、作业成果以及经验积累传承之间的高效衔接与复用,最大限度地降低装备运行成本、提高作业效率、保障海上作业安全。文章提出的载人潜水器运维体系能够为我国其他行业或部门的载人潜水器、远程缆控机器人(ROV)、水下自治机器人(AUV)等重大海洋装备的运行与保障提供有益参考借鉴。  相似文献   

导管架平台拆除过程稳定性及影响因素分析     

孙树峰  吕涛  陈国明  孙久洋  宋玉东  何睿 《海洋工程》2020,38(1):140-146

现有服役海洋平台正逐步走向老龄化,海洋平台拆除成为海洋工程新的经济增长点。针对达到服役年限的导管架平台,基于数值分析方法模拟拆除过程,开展平台在不同拆除阶段和环境载荷等参数下的稳定性及影响规律研究。基于钢结构整体稳定性理论,以导管架平台拆除过程中顶点位移为参考,定义导管架平台拆除作业稳定性指标,提出拆除作业阶段划分方法,通过分析导管架平台在不同影响因素作用下拆除过程稳定性响应,给出拆除作业建议。选取我国南海某4裙12腿进行实例分析,结果表明:主桩腿对导管架平台起决定性承载作用,拆除部分主桩腿后需要引入外部支撑力,以保证拆除作业安全进行;拆除构件越多,来浪方向和环境载荷大小对平台影响越加显著。研究结果可为导管架平台拆除作业提供参考。  相似文献   

基于GPU加速的Boussinesq类波浪传播变形数值模型     

孙家文  朱桐  房克照  刘忠波 《海洋工程》2020,38(2):111-119

Boussinesq波浪模型是一类相位解析模型,在时域内求解需要较高的空间和时间分辨率以保证计算精度。为提高计算效率,有必要针对该类模型开展并行算法的研究。与传统的中央处理器(CPU)相比,图形处理器(GPU)有大量的运算器,可显著提高计算效率。基于统一计算设备架构CUDA C语言和图形处理器,实现了Boussinesq模型的并行运算。将本模型的计算结果同CPU数值模拟结果和解析解相比较,发现得到的结果基本一致。同时也比较了CPU端与GPU端的计算效率,结果表明,GPU数值模型的计算效率有明显提升,并且伴随数值网格的增多,提升效果更为明显。  相似文献   

脚靴式海上升压站灌浆连接段强度分析研究   总被引：1，自引：1，他引：0  

王永发  孙震洲  汤群益  陈杰峰 《海洋工程》2020,38(5):107-115

与国内已建成的海上升压站相比，脚靴式海上升压站导管架对桩和套筒间的灌浆连接强度要求更高，完全套用单桩风电结构的分析方法会使结构设计不准确。由于桩和套筒之间设置有剪力键，当特殊情况时还需增加灌浆连接增强钢筋，在地震及撞船作用下容易导致灌浆接触界面分离等破坏，当前尚未有针对此类情况系统的灌浆连接强度校核方法。以某脚靴式海上升压站为例，分析其在在位、撞船、地震、疲劳等多个主控工况下灌浆连接、剪力键及增强钢筋的强度及稳定性，结果表明该海上升压站灌浆连接段满足设计要求，并研究了各参数对灌浆连接段强度的敏感程度，为今后脚靴式海上升压站各主控工况下的灌浆连接段强度分析、优化设计提供参考与借鉴。  相似文献   

KK型管节点应力集中系数几何敏感性分析     

林海花  孙承猛  石强 《海洋工程》2020,38(6):142-150

KK型管节点是自升式平台桁架式桩腿中的一种管节点，其应力集中系数是影响桩腿疲劳寿命的重要参数。应力集中系数与管节点的几何形式密切相关，为分析KK型管节点应力集中系数对几何参数的敏感性，利用ANSYS软件对某KK型管节点进行几何参数化建模，利用有限元数值模拟方法对各工况下的热点应力进行分析，并分别计算各相应工况下的名义应力，然后将热点应力与名义应力相比得到不同几何参数下的热点应力集中系数。对计算结果进行整理分析，得到了KK型管节点应力集中系数对无量纲几何参数的敏感性规律。结果表明，应力集中系数与撑杆受力状态、管节点结构形式有关，在满足结构布置、建造工艺和其他安全性指标的前提下，分析结果能够为KK型管节点的结构设计和疲劳分析提供技术支撑。  相似文献